Clemente, A., S.J. Chambers, F. Lodi, C. Nicoletti, G.M. Brett, 2004. Use of the indirect competitive ELISA for the detection of Brazil nut in food products. Food Control. 15:65–69.
Food related allergic reactions following inadvertent ingestion are increasingly common, with nuts, including Brazil nut, placed firmly in the top 10 food groups whose presence within a product should be declared. The presence of hidden allergens as a result of adulteration or contamination of ingredients presents a problem for both the food industry and the consumer. A sensitive and specific immunoassay for Brazil nut is described with a limit of detection of 1 ppm. Based upon the detection of the abundant 2S protein the assay is suitable for detection of raw and roasted Brazil nut in a range of food matrices.